User:Aram Kang/Notebook/Analysis of biofilm gene expression/2008/12/29

From OpenWetWare

< User:Aram Kang | Notebook | Analysis of biofilm gene expression | 2008 | 12(Difference between revisions)
Jump to: navigation, search
(Autocreate 2008/12/29 Entry for User:Aram_Kang/Notebook/Analysis_of_biofilm_gene_expression)
Current revision (06:00, 29 December 2008) (view source)
(Entry title)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Preparation of cell sample==
-
* Insert content here...
+
* innoculate a single colony from ECT501(for comparison) and three colonies from transformants(T1, 2, 3) into 5ml LB+kan liquid broth (at 3:30pm)
 +
* incubate 4 x 50ml tubes at 37°C with 250rpm for around 12-16hrs(less than 16hrs)
 +
* Tomorrow, before doing miniprep, I should innoculate liquid sample(maybe around 100ul) into another 5ml LB+kan

Current revision

Project name Main project page
Previous entry      

Preparation of cell sample

  • innoculate a single colony from ECT501(for comparison) and three colonies from transformants(T1, 2, 3) into 5ml LB+kan liquid broth (at 3:30pm)
  • incubate 4 x 50ml tubes at 37°C with 250rpm for around 12-16hrs(less than 16hrs)
  • Tomorrow, before doing miniprep, I should innoculate liquid sample(maybe around 100ul) into another 5ml LB+kan



Personal tools