User:Carly M. Montanero/Notebook/CHEM-571/2013/09/24: Difference between revisions
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'''Preparation of Pepsin-Hemoglobin Sample''' | '''Preparation of Pepsin-Hemoglobin Sample''' | ||
# Add 0.83 μL of pepsin to 4.99 ml of hemoglobin solution, creating a final concentration of 2 nM. | # Add 0.83 μL of pepsin to 4.99 ml of hemoglobin solution, creating a final concentration of 2 nM. | ||
# Incubate the tube at 37°C | # Incubate the tube at 37°C for 30 minutes. | ||
# Take aliquots every 30 minutes for 2 hours. | |||
'''Preparation of Pepstatin-Hemoglobin Sample''' | |||
# Add 0.83 μL of pepstatin to 4.99 ml of hemoglobin solution, creating a final concentration of 200 nM (0.2 μM). | |||
# Incubate the tube at 37°C for 30 minutes. | |||
# Take aliquots every 30 minutes for 2 hours. | # Take aliquots every 30 minutes for 2 hours. | ||
Revision as of 09:53, 24 September 2013
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ObjectiveTo observe the catalytic activity of pepsin (in cleaving peptide bonds in hemoglobin) in the presence and absence of pepstatin. This data will be compared with data we take in a later lab on pepsin-AuNPs. ProcedurePreparation of Pepsin-Hemoglobin Sample
Preparation of Pepstatin-Hemoglobin Sample
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