User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29: Difference between revisions
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==Procedure== | ==Procedure== | ||
'''Creating a Gold Stock Solution''' | |||
# | |||
'''Synthesizing Lysozyme AuNP and Myoglobin AuNP''' | '''Synthesizing Lysozyme AuNP and Myoglobin AuNP''' | ||
Solutions with the following Au:protein ratio and a final gold concentration of 0.25 mM were made using the information in Figure 1: | Solutions with the following Au:protein ratio and a final gold concentration of 0.25 mM were made using the information in Figure 1: | ||
''Gold to Lysozyme Ratio'' | |||
30 | :*30 | ||
50 | :*50 | ||
70 | :*70 | ||
90 | :*90 | ||
110 | :*110 | ||
130 | :*130 | ||
150 | :*150 | ||
170 | :*170 | ||
190 | :*190 | ||
210 | :*210 | ||
[[Gold to Myoglobin Ratio]] | [[Gold to Myoglobin Ratio]] | ||
10 | :*10 | ||
15 | :*15 | ||
25 | :*25 | ||
30 | :*30 | ||
35 | :*35 | ||
40 | :*40 | ||
45 | :*45 | ||
50 | :*50 | ||
# Make your samples in small test tubes and cap with aluminum foil. | |||
Make your samples in small test tubes and cap with aluminum foil | # The samples were heated at 80°C for 4 hours. | ||
Revision as of 09:36, 29 October 2013
 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAnalysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs. ProcedureCreating a Gold Stock Solution Synthesizing Lysozyme AuNP and Myoglobin AuNP Solutions with the following Au:protein ratio and a final gold concentration of 0.25 mM were made using the information in Figure 1: Gold to Lysozyme Ratio
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