User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Procedure== | ==Procedure== | ||
'''Creating the Gold Stock Solution''' | '''Creating the Gold Stock Solution''' | ||
# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O | # Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water. | ||
# The final concentration of gold stock was 2.57 mM. | # The final concentration of gold stock was 2.57 mM. | ||
'''Creating the Lysozyme Solution''' | '''Creating the Lysozyme Stock Solution''' | ||
# Add | # Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water. | ||
# The final concentration of lysozyme stock was 15 μM. | |||
'''Creating the Myoglobin Solution''' | '''Creating the Myoglobin Stock Solution''' | ||
# Add | # Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water. | ||
# The final concentration of myoglobin stock was 15 μM. | |||
'''Synthesizing Lysozyme AuNP and Myoglobin AuNP''' | '''Synthesizing Lysozyme AuNP and Myoglobin AuNP''' | ||
Solutions with the following Au:protein ratio were synthesized: | Solutions with the following Au:protein ratio were synthesized: | ||
''Gold to Lysozyme Ratio'' | :''Gold to Lysozyme Ratio'' | ||
:*30 | ::*30 | ||
:*50 | ::*50 | ||
:*70 | ::*70 | ||
:*90 | ::*90 | ||
:*110 | ::*110 | ||
:*130 | ::*130 | ||
:*150 | ::*150 | ||
:*170 | ::*170 | ||
:*190 | ::*190 | ||
:*210 | ::*210 | ||
''Gold to Myoglobin Ratio'' | :''Gold to Myoglobin Ratio'' | ||
:*10 | ::*10 | ||
:*15 | ::*15 | ||
:*25 | ::*25 | ||
:*30 | ::*30 | ||
:*35 | ::*35 | ||
:*40 | ::*40 | ||
:*45 | ::*45 | ||
:*50 | ::*50 | ||
# As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil. | # As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil. | ||
# The samples were heated at 80°C for 4 hours. | # The samples were heated at 80°C for 4 hours. | ||
==Figures== | |||
[[Image:10.29.2013_cmj_solutions1.png]] | |||
[[Image:10.29.2013_cmj_solutions2.png]] | |||
*Myoglobin UV-Vis | |||
[[Image:11.5.2013_cmj_UVVis_myo.png]] | |||
*Lysozyme UV-Vis | |||
[[Image:11.5.2013_cmj_UVVis_lyzo.png]] | |||
==Notes== | ==Notes== |
Revision as of 11:41, 12 November 2013
Biomaterials Design Lab: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAnalysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs. ProcedureCreating the Gold Stock Solution
Creating the Lysozyme Stock Solution
Creating the Myoglobin Stock Solution
Synthesizing Lysozyme AuNP and Myoglobin AuNP Solutions with the following Au:protein ratio were synthesized:
Figures
Notes
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