User:Carly M. Montanero/Notebook/CHEM-571/2013/11/20: Difference between revisions
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'''Preparing Invertase-AuNP Samples for UV-Vis''' | '''Preparing Invertase-AuNP Samples for UV-Vis''' | ||
# Centrifuge 1mL of the sample for 30 min at max rpm. | # Centrifuge 1mL of the sample for 30 min at max rpm. | ||
# Take UV-Vis measurements of the samples. | # Take UV-Vis measurements of the samples.] | ||
Solutions with the following Au:protein ratio were run on the UV-Vis: | Solutions with the following Au:protein ratio were run on the UV-Vis: | ||
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'''Creating the Gold Stock Solution''' | '''Creating the Gold Stock Solution''' | ||
# Add 0.0562 g of | # Add 0.0562 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water. | ||
# The final concentration of gold stock was 2.85 mM. | # The final concentration of gold stock was 2.85 mM. | ||
'''Creating the BSA Stock Solution''' | '''Creating the BSA Stock Solution''' | ||
# Add 0. | # Add 0.0260 g of BSA to a 25 mL volumetric flask and fill with distilled water. | ||
# The final concentration of BSA stock was | # The final concentration of BSA stock was 15.65 μM. | ||
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# As seen below, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil. | # As seen below, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil. | ||
# The samples were heated at 80°C for 4 hours. | # The samples were heated at 80°C for 4 hours. | ||
[[Image:11.20.2013_cmj_solutions1.png]] | |||
==Figures== | |||
[[Image:11.20.2013_cmj_Invertase_UVvis.png]] | |||
Revision as of 11:02, 3 December 2013
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ObjectiveTo synthesize BSA-AuNPs and run UV-Vis on the invertase-AuNP solutions created yesterday. ProcedurePreparing Invertase-AuNP Samples for UV-Vis
Figures
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