Objective
To synthesize invertase-AuNPs and BSA-AuNPs.
Procedure
Creating the Gold Stock Solution
- Add 0.0345 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
- The final concentration of gold stock was 3.59 mM.
Creating the Invertase Stock Solution
- Add 0.1015 g of invertase to a 50 mL volumetric flask and fill with distilled water.
- The final concentration of invertase stock was 30.07μM
Creating the BSA Stock Solution
- Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.
- The final concentration of BSA stock was 21.8 mM.
Solutions with the following Au:protein ratio were synthesized:
- Gold to Invertase Ratio
- 30
- 50
- 70
- 90
- 110
- 130
- 150
- 170
- 190
- 210
- Gold to BSA Ratio
- 105
- 110
- 115
- 120
- 125
- 130
- 135
- 140
- 145
- 150
- As seen below, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
- The samples were heated at 80°C for 4 hours.
Figures
Notes
- The BSA stock concentration was calculated incorrectly. We will remake the BSA-AuNP solutions tomorrow.
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