User:Carly M. Montanero/Notebook/CHEM-571/2014/01/29: Difference between revisions

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==Procedure==
==Procedure==
# Shake each test tube (40, 50, 110 ratio) of lysozyme-AuNP until fibers appear to be evenly distributed throughout.
# Shake each test tube (50, 110 ratio) of lysozyme-AuNP until fibers appear to be evenly distributed throughout.
# Take 1 mL aliquots from each test tube and place in 1.5 mL tubes.  
# Take 1 mL aliquots from each test tube and place in 1.5 mL tubes.  


Revision as of 09:55, 29 January 2014

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Objective

To test fiber dissolution by adding various chelators and changing the pH.

Procedure

  1. Shake each test tube (50, 110 ratio) of lysozyme-AuNP until fibers appear to be evenly distributed throughout.
  2. Take 1 mL aliquots from each test tube and place in 1.5 mL tubes.

Chelators

Creating Stock Solutions of Chelators

File:1.29.data.chelator.stock

Adding Chelators to Lysozyme-AuNP Solutions

  1. Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.
  2. Record effects of each chelator on each solution.

pH

Creating 1 M Buffer Solutions

Citric Acid

  1. Add 42.02 g of citric acid in 180 mL distilled water.
  2. Titrate to pH 3.0 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

Phosphate

  1. Add 0.0908 mol of sodium phosphate monobasic to 0.1091 mol of sodium phosphate dibasic.
  2. Fill up to 200 mL with distilled water.

Tris

  1. Dissolve 24.228 g Tris base in 180 mL distilled water.
  2. Titrate to pH 9.0 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

MES

  1. Dissolve 39.04 g MES in 180 mL distilled water.
  2. Titrate to pH 5.2 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

HEPES

  1. Dissolve 47.66 g HEPES in 180 mL distilled water.
  2. Titrate to pH 7 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

CHES

  1. Dissolve 41.46 g CHES in 180 mL distilled water.
  2. Titrate to pH 9 with monovalent strong acid/base if necessary.
  3. Fill up to 200 mL with distilled water.

Adding Buffers to Lysozyme-AuNP Solutions

  1. Dilute the 1 M solutions to 100 mM, 10 mM, and 1 mM.
  2. Add 1 mL of each to the lysozyme-AuNP solutions.
  3. Let sit overnight and record results.


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