User:Carly M. Montanero/Notebook/CHEM-571/2014/02/04: Difference between revisions

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'''Creating Stock Solutions of Chelators'''
'''Creating Stock Solutions of Chelators'''


[[Image:1.29.data.chelator.stock]]
[[Image:1.29.data.chelator.png|800px|




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# Record effects of each chelator on each solution.  
# Record effects of each chelator on each solution.  


[[Image:1.29.data.chelator.add]]
[[Image:1.29.data.chelator.add|800px|]]


===pH===
===pH===

Revision as of 12:59, 4 February 2014

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Objective

  • To create stock solutions of chelators.
  • Add the various chelators to lysozyme-AuNP solutions.
  • Change pH of lysozyme-AuNP solutions.
  • Record results.

Procedure

Chelators

Creating Stock Solutions of Chelators

[[Image:1.29.data.chelator.png|800px|


Adding Chelators to Lysozyme-AuNP Solutions

  1. Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.
  2. Record effects of each chelator on each solution.

pH

Adding Buffers to Lysozyme-AuNP Solutions

  1. Dilute the 1 M solutions to 100 mM, 10 mM, and 1 mM.
  2. Add 1 mL of each to the lysozyme-AuNP solutions.
  3. Let sit overnight and record results.


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