Flourescence
Procedure
- Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 1.0mM HCl 3mL total
- Au 4.57mM
- (0.25mM)(3.0mL)=(XmL)(4.57mM)
- XmL= .176mL= 176uL Au
- Myoglobin 160uM
- (12.5uM)(3.0mL)=(XmL)(160uM)
- X=0.234mL= 234uL Myoglobin
- HCl 1.0mM
- (3.0mL)(1.0mM)=(1.0mM)(XmL)
- XmL=3.0mL= 300uL HCl
- Water
- Volume of water= 3000uL- 176uL-234uL-300uL= 2290uL
- Absorbance measurements were taken and saved every 3 minutes for 3 hours
- The instrument was set at 80 degrees Celsius
- The scan range parameters were start at 310nm and ending at 540nm
- The emission and exit slit were set at 10nm
UV-Vis Solutions
Stock Concentrations:
- Au 4.57mM
- Myoglobin 160mM
- Fructose 40.9mM
0.25 mM Fructose
pH
|
μL of 1 mM HClstock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AuCl3stock
|
μL of Fructosestock
|
μL of Myoglobinstock
|
μL of Water
|
4 |
500 |
0 |
0 |
293 |
31 |
388 |
3788
|
5 |
50 |
0 |
0 |
293 |
31 |
388 |
4238
|
6 |
5 |
0 |
0 |
293 |
31 |
388 |
4283
|
7 |
0 |
0 |
0 |
293 |
31 |
388 |
4288
|
8 |
0 |
5 |
0 |
293 |
31 |
388 |
4283
|
9 |
0 |
50 |
0 |
293 |
31 |
388 |
4238
|
10 |
0 |
500 |
0 |
293 |
31 |
388 |
3788
|
11 |
0 |
0 |
5 |
293 |
31 |
388 |
4283
|
12 |
0 |
0 |
50 |
293 |
31 |
388 |
4238
|
0.75 mM Fructose
pH
|
μL of 1 mM HClstock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AuCl3stock
|
μL of Fructosestock
|
μL of Myoglobinstock
|
μL of Water
|
4 |
500 |
0 |
0 |
293 |
92 |
388 |
3727
|
5 |
50 |
0 |
0 |
293 |
92 |
388 |
4177
|
6 |
5 |
0 |
0 |
293 |
92 |
388 |
4222
|
7 |
0 |
0 |
0 |
293 |
92 |
388 |
4227
|
8 |
0 |
5 |
0 |
293 |
92 |
388 |
4222
|
9 |
0 |
50 |
0 |
293 |
92 |
388 |
4177
|
10 |
0 |
500 |
0 |
293 |
92 |
388 |
3727
|
11 |
0 |
0 |
5 |
293 |
92 |
388 |
4222
|
12 |
0 |
0 |
50 |
293 |
92 |
388 |
4177
|
1.25 mM Fructose
pH
|
μL of 1 mM HClstock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AuCl3stock
|
μL of Fructosestock
|
μL of Myoglobinstock
|
μL of Water
|
4 |
500 |
0 |
0 |
293 |
159 |
388 |
3666
|
5 |
50 |
0 |
0 |
293 |
159 |
388 |
4116
|
6 |
5 |
0 |
0 |
293 |
159 |
388 |
4161
|
7 |
0 |
0 |
0 |
293 |
159 |
388 |
4166
|
8 |
0 |
5 |
0 |
293 |
159 |
388 |
4161
|
9 |
0 |
50 |
0 |
293 |
159 |
388 |
4116
|
10 |
0 |
500 |
0 |
293 |
159 |
388 |
3666
|
11 |
0 |
0 |
5 |
293 |
159 |
388 |
4161
|
12 |
0 |
0 |
50 |
293 |
159 |
388 |
4116
|
Results
After the sample was run through fluorescence, no color change had occurred indicating there was a source of error in the data collected, possibly from there being no gold in the sample prepared or from a too high concentration of protein being used. The graphs for the incorrect data were still made and are shown below. The correct sample was run on 09/29/2016 by Matt Hartings and the correct graphs were created.
Incorrect Graphs
Integrated intensity for Myoglobin templated Gold Nanoparticles at pH4
Maximum intensity for Myoglobin templated Gold Nanoparticles at pH4
Maximum Wavelength for Myoglobin templated Gold Nanoparticles at pH4
- The graphs for the correct data were created by Matt Hartings. Data was collected and interpreted on 09/28/2016
User:Matt Hartings/Notebook/AU Biomaterials Design Lab
|