User:Daniel Goodman/Notebook/Cluzel/2010/03/29: Difference between revisions
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== | ==Prepare for Afternoon/Evening== | ||
===Agarose=== | |||
4% agarose, 4.5 ml, 0.18 mg low melt agarose | |||
heat at 80 degrees | |||
(put in heat bath at 1:00) | |||
===Cells=== | |||
Put one GFP colony from last week's plate in culture flash w/ 4ml LB, 4ul amp (shaker at 12:50pm) | |||
===Chips=== | |||
Clean chips in hot water DI bath, 80 degrees | |||
(put in heat bath at 1:00) | |||
===Strains=== | |||
Aliquot from Mark Kim's EZR-12-CFP strain, nanodrop, prepare cloning for tonight | |||
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__NOTOC__ | __NOTOC__ | ||
Latest revision as of 10:02, 29 March 2010
 Cluzel Lab Notebook Daniel Goodman |
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Prepare for Afternoon/EveningAgarose4% agarose, 4.5 ml, 0.18 mg low melt agarose heat at 80 degrees (put in heat bath at 1:00) CellsPut one GFP colony from last week's plate in culture flash w/ 4ml LB, 4ul amp (shaker at 12:50pm) ChipsClean chips in hot water DI bath, 80 degrees (put in heat bath at 1:00) StrainsAliquot from Mark Kim's EZR-12-CFP strain, nanodrop, prepare cloning for tonight | ||
