User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/18: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Fate Switch by Synthetic Transcription Factors</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Fate Switch by Synthetic Transcription Factors</span>
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==ELISA==
==ELISA==
===Data Analysis===
====Data Analysis=====
* Experiment showed no concentration of Insulin or Glucagon (low absorbance)
* Experiment showed no concentration of Insulin or Glucagon (low absorbance)
* Control experiment (only substrate) turned very blue (high absorbance)
* Control experiment (only substrate) turned very blue (high absorbance)
&nbsp;&nbsp;&nbsp; Added TBST in last washing step - activation of substrate?
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Added TBST in last washing step - activation of substrate?
 
==Bioinformatics==
====GO Term Analysis====
* Should go over GO Terms again
* G-protein linked to secretion, acceptance of glucagon/insulin (importance)
* What GO Terms should we see?
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; GSIS, Secretion, Packaging, Membrane Permeation Integral Proteins...




Latest revision as of 00:31, 27 September 2017

Cell Fate Switch by Synthetic Transcription Factors Main project page
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Pancreatic Cell Culture

  • Insert content here...

RT-PCR

  • Protocol
  • Note on bench to make 750nM primers

ELISA

Data Analysis=

  • Experiment showed no concentration of Insulin or Glucagon (low absorbance)
  • Control experiment (only substrate) turned very blue (high absorbance)

           Added TBST in last washing step - activation of substrate?

Bioinformatics

GO Term Analysis

  • Should go over GO Terms again
  • G-protein linked to secretion, acceptance of glucagon/insulin (importance)
  • What GO Terms should we see?

           GSIS, Secretion, Packaging, Membrane Permeation Integral Proteins...



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