User:Dhea Patel/Notebook/Hemoglobin Project/2013/06/13: Difference between revisions
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<b>Mb/Tris in Acetone, Trial 2</b> | <b>Mb/Tris in Acetone, Trial 2</b> | ||
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The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned. | The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned. | ||
Revision as of 09:10, 13 June 2013
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Reaction of OPD+H2O2 with Mb Catalyst in Acetone SuspensionThe procedurefor the reaction of OPD and H2O2 was followed using a Mb/Tris catalyst and an acetone suspension. The reaction was run in a beaker with a stir bar and the solution was centrifuged for 1 minute at 13,200 rpm before taking a spectrum. In addition reactions were run using Mb/Phosphate and Mb/Acetate in hexanes suspension. These samples were filtered (not centrifuged) before taking a spectrum. DataThe drop in absorbance at 140 minutes is due to the outside of the cuvette being cleaned. Mb/Tris in Acetone, Trial 2 The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned. |