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Objective
- Make 40% Beads
- Filter All Beads for drying
- Vacuum Filtrate and grind up 25% and 40% yellow beads
- Sieve dried yellow beads
Description
- 40% Beads: Used procedure from first day
- 2.5g PVOH, 54 mL bentonite solution, 26mL water, 30mL HCl
- Do two rounds of 150mL ethyl acetate (half of bead solution in one)
- For each round put in 1.5mL glutaraldehyde twice (6mL total)
- Vacuum Filtrated 25% and 40% beads.
- 25% Beads: 1.2262g
- 40% Beads: 1.8708g
- Ground beads using mortal and pestle
- Left to dry overnight
- Vacuum Filtrated New Beads
- Placed on watch-glass to dry
Data
- Calculated Swelling using masses right after freeze drying and 'wet' masses after letting them sit in ethyl acetate/water solution for a week and then vacuum filtrating. Swelling % = (wet-dry)/dry
- 10%: 60.9%
- 25%: 21.8%
- 40%: 66.6%
Notes
- Cabbage Dye is natural and therefore susceptible to mold and to going bad
- Cabbage left in drawer and not refrigerated went bad and changed colors.
- Will have to make new dye and keep it in fridge this time.
- Any experiments using dye will have to be done earlier rather than later to avoid dye going bad.
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Biomaterials Design Lab
