User:Dominique Brager/Notebook/481 Notebook/2017/02/10

Biomaterials Design Lab

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Objective Make more beads Run UV-Vis for Red Cabbage Dye at different pH Description Make more beads using procedure from Day 1 Double recipe and use all solution (probably about 100mL total) Made 10% first 2.5g PVOH, 30mL HCl, 70mL water, 10mL bentonite solution 200mL Ethyl Acetate 6mL glutaryaldehyde Did not use enough ethyl acetate (will repeat and increase to 300 mL if beads do not turn out will) Make 25% 2.5g PVOH, 30mL HCL, 54mL water, 26mL bentonite solution Same Ethyl Acetate and glutaraldehyde as 10% Made more 3% bentonite solution Run UV-Vis on dye at different pH pH 1 Dye is reddish pink Made dilutions Stock solution absorbance was way too high so made dilution of 1:9 Dye to Water. Used 1:9 Dye to Water (Stock 2.0) to make dilutions of 4:1 stock 2.0 to water, 3:2, 2:3, and 1:4. Dye absorbed at 510nm Made trendline: Tried sonicating beads from last week Did not work Vacuum Filtrated Beads from last week Mass of 10% beads after vacuum filtration: 1.3045g Data Add data and results here... Notes Dilutions made last class with dye and water changed colors. Stock solution stayed purple. Dilutions turned more blue. (this indicated that they turned more basic)