User:Elaine Marie Robbins/Notebook/CHEM-496/2011/08/31: Difference between revisions
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==Objective== | ==Objective== | ||
To determine how pH affects the synthesis of gold nanoparticles ( | To determine how pH affects the synthesis of gold nanoparticles (AuNP) using the procedure developed here: | ||
Revision as of 09:49, 19 October 2011
Synthesizing Gold Nanoparticles | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo determine how pH affects the synthesis of gold nanoparticles (AuNP) using the procedure developed here:
http://pubs.acs.org/doi/abs/10.1021/jp110296y Description
DataCalculations:
The absorbance values for the acetate buffer were subtracted from absorbance values for the reaction mixture recorded by the UV-vis to find a corrected absorbance value that would indicate the absorbance of the AuNP and the BSA only. The corrected absorbance values at 550 nm (The wavelength that Au NP should absorb) were graphed against time. The graph (below) shows that some AuNP were created, as absorbance values do increase. However, it is possible some were destroyed as the absorbance rates do drop slightly starting at 120 min into the procedure.
NotesThe reaction mixture remained clear for most of the experiment, until towards the end when a slight yellowish tinge appeared. Bashki, et al indicated that the mixture should be purplish in color. This could indicate that the reaction did not occur properly and may need to be repeated.
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