User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/18: Difference between revisions
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==Description== | ==Description== | ||
<b>Fluorophore</b> | <b>Fluorophore</b> | ||
# Combine 2 mg of 5-iodoacetamido fluorescein (5-IAF) with | # Combine 2 mg of 5-iodoacetamido fluorescein (5-IAF) with excess cysteine (0.39 mg) in 3.95 mL of 50 mM Tris buffer (pH 7.5). | ||
# | # Vortex to mix. | ||
# Wrap reaction tube in foil and allow reaction to proceed for 2 hr. | |||
# Add 100 μL of dithiothreitol (DTT) | |||
==Data== | ==Data== | ||
<b>Conductivity of AuNP II</b> | |||
The Pd plates broke off of the Teflon plate, so the test was not conducted. | |||
==Notes== | ==Notes== | ||
Fluorophore and references used in this experiment: | |||
http://www.markergene.com/ProductDetails.php/M0638 | |||
Revision as of 10:16, 19 October 2011
Conductivity of AuNP III, Fluorophore | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveConductivity of AuNP III To run the conductive plates made on 10/12/11 to determine the conductivity of AuNP.
To make a labeled cysteine fluorophore. DescriptionFluorophore
DataConductivity of AuNP II The Pd plates broke off of the Teflon plate, so the test was not conducted. NotesFluorophore and references used in this experiment: http://www.markergene.com/ProductDetails.php/M0638
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