User:Eleni N. Kalivas/Notebook/CHEM-571/2013/08/28: Difference between revisions
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*Using Beer's Law the experimental concentration was also determined | *Using Beer's Law the experimental concentration was also determined | ||
[[Image:Chart 1 (1).png | | [[Image:Chart 1 (1).png | 300px]] | ||
Observations/Results: The sodium citrate was adding to the HAuCl4 while it was still boiling and stirring. Immediately after adding the sodium nitrate the solution turned a light grey then began to slowly get darker and take on a dark purple tone. Once it seemed to be reach a very deep purple it then began to lighten up and become somewhat red. After 40 minutes the solution was left to cool down to room temperature and using a graduated cylinder the final volume was measured to be 9.5mL, which resulted in a final theoretical concentration of HAuCl4 was 0.0054M and of the sodium nitrate was 0.0013 M. | Observations/Results: | ||
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*The sodium citrate was adding to the HAuCl4 while it was still boiling and stirring. Immediately after adding the sodium nitrate the solution turned a light grey then began to slowly get darker and take on a dark purple tone. Once it seemed to be reach a very deep purple it then began to lighten up and become somewhat red. After 40 minutes the solution was left to cool down to room temperature and using a graduated cylinder the final volume was measured to be 9.5mL, which resulted in a final theoretical concentration of HAuCl4 was 0.0054M and of the sodium nitrate was 0.0013 M. | |||
After analyzing the graph the peak was not within the typical range, from this it can be concluded that the 1/10th dilution of the stock solution of citrate-AuNP does not have enough gold material which is effecting the absorption peak. Therefore we have decided to make a new dilution from the stock solution and re-run the sample on the UV-Vis. | After analyzing the graph the peak was not within the typical range, from this it can be concluded that the 1/10th dilution of the stock solution of citrate-AuNP does not have enough gold material which is effecting the absorption peak. Therefore we have decided to make a new dilution from the stock solution and re-run the sample on the UV-Vis. | ||
Revision as of 09:26, 18 September 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectivesUse two different ways to synthesize gold nanoparticles. The first method requires the use of a protein called bovine serum albumin (BSA). This protein reduces the Au3+ and also encloses and stabilizes the gold nanoparticles. The second method includes using sodium citrate. The sodium citrate also reduces the Au3+ and also encloses and stabilizes the gold nanoparticles. BSA-AuNPProtocol:
Citrate-AuNPProtocol:
After analyzing the graph the peak was not within the typical range, from this it can be concluded that the 1/10th dilution of the stock solution of citrate-AuNP does not have enough gold material which is effecting the absorption peak. Therefore we have decided to make a new dilution from the stock solution and re-run the sample on the UV-Vis. Matt Hartings . Also, use the reference on my page in order to determine what the concentration of citrate-AuNPs are.
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