User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/03

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Objective

  • determine the molar absorptivity of adenosine and inosine.
  • these two molecule's functionality differ in that adenosine contains a primary amine while inosine contains a carboxy group
  • Analyze changes in the UV-vis spectra to determine changes in the concentration.
  • use Beer's law to determine the molar absorptivity, and create a calibration curve

Once each group has created a calibration curve we will then determine the standard deviations and the confidence interval, and lastly we will perform a Q-test in order to remove any outlining data.

Adenosine Protocol

Using 0.2006 grams of adenosine and a 50mL volumetric a stock solution of 0.015M adenosine solution was prepared. The stock solution was then diluted to 1.5e-4 M solution, which was used to create the following dilutions which was prepared to run on the UV-vis.

Adenosine solution concentrations (M)
3.00x10-5
2.50x10-5
2.00x10-5
1.50x10-5
1.00x10-5
0.50x10-5
RANDOM
  • The data was then analyzed for corrections which will be done tomorrow.

Inosine Protocol

Using 0.1347 grams of inosine and a 25mL volumetric a stock solution of 0.02M adenosine solution was prepared. The stock solution was then diluted to 4.8e-4 M solution, which was used to create the following dilutions which was prepared to run on the UV-vis.

Inosine solution concentrations (M)
4.80x10-5
4.00x10-5
3.20x10-5
2.40x10-5
1.60x10-5
0.80x10-5
RANDOM
  • Due to time constraints there was not enough time to run these samples, they will be tested tomorrow.



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