User:Eleni N. Kalivas/Notebook/CHEM-571/2014/02/04: Difference between revisions
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# Remove 1 mL aliquot from test tube and place in the cuvette. | # Remove 1 mL aliquot from test tube and place in the cuvette. | ||
# Take the UV-Vis spectra of the sample. | # Take the UV-Vis spectra of the sample. | ||
#Take the fluorescence spectra of each sample | |||
# Repeat for each test tube | # Repeat for each test tube | ||
# Take the UV-Vis spectra of each standard: BSA, L-cysteine, zinc (II) nitrate and manganese (II) acetate. | # Take the UV-Vis spectra of each standard: BSA, L-cysteine, zinc (II) nitrate and manganese (II) acetate. | ||
# Data analysis of the UV-Vis absorbances | # Data analysis of the UV-Vis absorbances, and fluorescence spectra | ||
# Determine if any changes in procedure need to be made. | # Determine if any changes in procedure need to be made. | ||
## Is a different scale of protein:ligand ratio required? | ## Is a different scale of protein:ligand ratio required? | ||
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Note: Much of the protocol for today will be determined by the results of our first synthesis. | Note: Much of the protocol for today will be determined by the results of our first synthesis. | ||
== | ==Edited Protocol== | ||
* | * we are going to increase the final volume of solution to yield more mass to be collected. The original final volume will be multiplied by three. | ||
Corrected Volumes Table | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''% BSA''' | |||
| align="center" style="background:#f0f0f0;"|'''5 mM Zinc Nitrate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5mM Manganese Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''40 mM Ammonium Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''10uM BSA* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''10uM L-Cysteine (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''10mM Ammonium Hydroxide (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5 mM Sodium Sulfide* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)''' | |||
|- | |||
| 0||540||60||600||0||600||3000||600||5.4 | |||
|- | |||
| 10||540||60||600||60||540||3000||600||5.4 | |||
|- | |||
| 20||540||60||600||120||480||3000||600||5.4 | |||
|- | |||
| 30||540||60||600||180||420||3000||600||5.4 | |||
|- | |||
| 40||540||60||600||240||360||3000||600||5.4 | |||
|- | |||
| 50||540||60||600||300||300||3000||600||5.4 | |||
|- | |||
| 60||540||60||600||360||240||3000||600||5.4 | |||
|- | |||
| 70||540||60||600||420||180||3000||600||5.4 | |||
|- | |||
| 80||540||60||600||480||120||3000||600||5.4 | |||
|- | |||
| 90||540||60||600||540||60||3000||600||5.4 | |||
|- | |||
| 100||540||60||600||600||0||3000||600||5.4 | |||
|} | |||
==Notes== | ==Notes== | ||
Add before and after photos, and get uv-light photo. | |||
<br>UV-Vis of BSA-L-Cysteine | |||
[[Image:Bsa-cys.png|750px ]] | |||
Revision as of 18:51, 15 April 2014
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ObjectiveCharacterize the previously synthesized BSA-L-cysteine ZnS:Mn Nanocrystals using UV-Vis. Protocol
Note: Much of the protocol for today will be determined by the results of our first synthesis. Edited Protocol
NotesAdd before and after photos, and get uv-light photo.
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