User:Eleni N. Kalivas/Notebook/CHEM-571/2014/02/04

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Objective

Characterize the previously synthesized BSA-L-cysteine ZnS:Mn Nanocrystals using UV-Vis.

Protocol

  1. Remove 1 mL aliquot from test tube and place in the cuvette.
  2. Take the UV-Vis spectra of the sample.
  3. Repeat for each test tube
  4. Take the UV-Vis spectra of each standard: BSA, L-cysteine, zinc (II) nitrate and manganese (II) acetate.
  5. Data analysis of the UV-Vis absorbances
  6. Determine if any changes in procedure need to be made.
    1. Is a different scale of protein:ligand ratio required?
    2. Were QD NCs successfully synthesized?
  7. Prepare stock solutions for next synthesis on the following day.

Note: Much of the protocol for today will be determined by the results of our first synthesis.

Data

  • UV-Vis


  • Fluorescence

Notes

Add before and after photos, and get uv-light photo.




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