Objective
Characterize the previously synthesized BSA-L-cysteine ZnS:Mn Nanocrystals using UV-Vis.
Protocol
- Remove 1 mL aliquot from test tube and place in the cuvette.
- Take the UV-Vis spectra of the sample.
- Take the fluorescence spectra of each sample
- Repeat for each test tube
- Take the UV-Vis spectra of each standard: BSA, L-cysteine, zinc (II) nitrate and manganese (II) acetate.
- Data analysis of the UV-Vis absorbances, and fluorescence spectra
- Determine if any changes in procedure need to be made.
- Is a different scale of protein:ligand ratio required?
- Were QD NCs successfully synthesized?
- Prepare stock solutions for next synthesis on the following day.
Note: Much of the protocol for today will be determined by the results of our first synthesis.
Edited Protocol
- we are going to increase the final volume of solution to yield more mass to be collected. The original final volume will be multiplied by three.
Corrected Volumes Table
% BSA
|
5 mM Zinc Nitrate (uL)
|
5mM Manganese Acetate (uL)
|
41.65 mM Ammonium Acetate (uL)
|
11uM BSA* (uL)
|
10uM L-Cysteine (uL)
|
10mM Ammonium Hydroxide (uL)
|
5 mM Sodium Sulfide* (uL)
|
Total Volume (mL)
|
0 |
540 |
60 |
600 |
0 |
600 |
3000 |
600 |
5.4
|
10 |
540 |
60 |
600 |
60 |
540 |
3000 |
600 |
5.4
|
20 |
540 |
60 |
600 |
120 |
480 |
3000 |
600 |
5.4
|
30 |
540 |
60 |
600 |
180 |
420 |
3000 |
600 |
5.4
|
40 |
540 |
60 |
600 |
240 |
360 |
3000 |
600 |
5.4
|
50 |
540 |
60 |
600 |
300 |
300 |
3000 |
600 |
5.4
|
60 |
540 |
60 |
600 |
360 |
240 |
3000 |
600 |
5.4
|
70 |
540 |
60 |
600 |
420 |
180 |
3000 |
600 |
5.4
|
80 |
540 |
60 |
600 |
480 |
120 |
3000 |
600 |
5.4
|
90 |
540 |
60 |
600 |
540 |
60 |
3000 |
600 |
5.4
|
100 |
540 |
60 |
600 |
600 |
0 |
3000 |
600 |
5.4
|
Notes
Add before and after photos, and get uv-light photo.
|