User:Eleni N. Kalivas/Notebook/CHEM-571/2014/02/05: Difference between revisions
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#Synthesize new nano crystals using new ligand GSH | #Synthesize new nano crystals using new ligand GSH | ||
#Attempt new synthesis method described below | #Attempt new synthesis method described below | ||
Preparation of BSA-GSH nanocrystal solutions | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''% BSA''' | |||
| align="center" style="background:#f0f0f0;"|'''5 mM Zinc Nitrate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5mM Manganese Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''41.65 mM Ammonium Acetate (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''11uM BSA* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''10uM GSH (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''10mM Ammonium Hydroxide (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''5 mM Sodium Sulfide* (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)''' | |||
|- | |||
| 0||540||60||600||0||600||3000||600||5.4 | |||
|- | |||
| 10||540||60||600||60||540||3000||600||5.4 | |||
|- | |||
| 20||540||60||600||120||480||3000||600||5.4 | |||
|- | |||
| 30||540||60||600||180||420||3000||600||5.4 | |||
|- | |||
| 40||540||60||600||240||360||3000||600||5.4 | |||
|- | |||
| 50||540||60||600||300||300||3000||600||5.4 | |||
|- | |||
| 60||540||60||600||360||240||3000||600||5.4 | |||
|- | |||
| 70||540||60||600||420||180||3000||600||5.4 | |||
|- | |||
| 80||540||60||600||480||120||3000||600||5.4 | |||
|- | |||
| 90||540||60||600||540||60||3000||600||5.4 | |||
|- | |||
| 100||540||60||600||600||0||3000||600||5.4 | |||
|} | |||
==Protocol for New synthesis method== | ==Protocol for New synthesis method== |
Revision as of 12:13, 5 February 2014
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Protocol
Preparation of BSA-GSH nanocrystal solutions
Protocol for New synthesis methodThis protocol was taken from Matt Harting's notebook
Notes
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