User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/08/31: Difference between revisions
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==Objective== | ==Objective== | ||
To synthesize gold nanoparticles (AuNPs) from bovine serum albumen (BSA) and HAuCl<sub>4</sub> | |||
==Description== | ==Description== | ||
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__NOTOC__ | __NOTOC__ | ||
# Create a 10 mL aqueous mixture of both BSA (0.0015 - 0.015 mM) and HAuCl<sub>4</sub> (0.25 - 1.0 mM) | # Create a 10 mL aqueous mixture of both BSA (0.0015 - 0.015 mM) and HAuCl<sub>4</sub> (0.25 - 1.0 mM) | ||
# Heat the mixture at 80°C for 4 hours in a water bath. | # Heat the mixture at 80°C for 4 hours in a water bath. | ||
#Twice an hour use a UV-vis Spectrometer to assess the results of the reaction. Use a wavelength range between 200 and 900 nm. The reaction reaches a maximum intensity when the peak is at 550 nm. | #Twice an hour use a UV-vis Spectrometer to assess the results of the reaction. Use a wavelength range between 200 and 900 nm. The reaction reaches a maximum intensity when the peak is at 550 nm. | ||
Revision as of 10:08, 31 August 2011
 Experimental Biological Chemistry
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ObjectiveTo synthesize gold nanoparticles (AuNPs) from bovine serum albumen (BSA) and HAuCl4 DescriptionData
NotesThis area is for any observations or conclusions that you would like to note.
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- Create a 10 mL aqueous mixture of both BSA (0.0015 - 0.015 mM) and HAuCl4 (0.25 - 1.0 mM)
- Heat the mixture at 80°C for 4 hours in a water bath.
- Twice an hour use a UV-vis Spectrometer to assess the results of the reaction. Use a wavelength range between 200 and 900 nm. The reaction reaches a maximum intensity when the peak is at 550 nm.
