User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/03/07

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Objective

To lower the pH of the dye reactions (to stop the reactions) and to perform dialysis on our samples.

Description

  1. Lower the pH of the BSA/HCl control with dye to 6.59 by adding 1μL of 1 M HCl. The solution became pink.
  2. Lower the pH of the 166 molar ratio with dye to 5.53 by adding 37 μL of 1 M HCl. The solution became light pink.
  3. Lower the pH of the 70 molar ratio with dye to 6.83 by adding 5 μL of 1 M HCl. The solution became purple.
  4. The solutions were transferred to Fisherbrand regenerated cellulose dialysis tubing. (flat width = 19 mm, vol/cm = 1.15 mL, wall thickness = 25μm, and dry cylinder diameter = 12.1 mm)
  5. The dialysis tubing containing the solutions were placed in a dialysis container filled with water and covered with aluminum foil.
  6. Over the course of the following week, the water was changed 7 times.

Data

  • No data was collected today

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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