User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/17: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 41: Line 41:
| 72 hr control||34.2||315.8
| 72 hr control||34.2||315.8
|}
|}
* I am student A, Sunny is student B. Therefore, the tubes are divided as follows:


==Part Two: 8% Acrylamide Gel==
==Part Two: 8% Acrylamide Gel==

Revision as of 12:55, 17 March 2009

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Experimental Goals

  1. Perform an Immunoprecipitation of iNOS/NOS2 from LPS +/- IFNg stimulated cell lysates.
  2. Preparation of 8% PAGE running gel.

Part One: IP of iNOS/NOS2

  • Volumes of RIPA buffer required for IP. (See previous entry for calculation of volumes.)
Sample Volume of sample required (µL) Volume of RIPA buffer needed (µL)
24 hr (+) LPS 64.9 285.1
48 hr (+) LPS 74.7 275.3
72 hr (+) LPS 97.9 252.1
24 hr (+) IFNg 34.9 315.1
48 hr (+) IFNg 40.3 309.7
72 hr (+) IFNg 350.0 0.0
24 hr (+) LPS (+) IFNg 92.6 257.4
48 hr (+) LPS (+) IFNg 128.8 221.2
72 hr (+) LPS (+) IFNg 62.5 287.5
24 hr control 34.2 315.8
48 hr control 35.6 314.4
72 hr control 34.2 315.8
  • I am student A, Sunny is student B. Therefore, the tubes are divided as follows:

Part Two: 8% Acrylamide Gel


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Eric_Ma/Notebook/MICB323_Lab_Book/2009/03/17&oldid=294553"