User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/17: Difference between revisions

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==Part One: IP of iNOS/NOS2==
==Part One: IP of iNOS/NOS2==
* Volumes of RIPA buffer required for IP. (See previous entry for calculation of volumes.)
* Volumes of RIPA buffer required for IP. (See previous entry for calculation of volumes.)
* I am student A, Sunny is Student B, therefore the samples are divided as follows:
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Experimenter'''
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''Volume of sample required (µL)'''
| align="center" style="background:#f0f0f0;"|'''Volume of sample required (µL)'''
| align="center" style="background:#f0f0f0;"|'''Volume of RIPA buffer needed (µL)'''
| align="center" style="background:#f0f0f0;"|'''Volume of RIPA buffer needed (µL)'''
|-
|-
| 24 hr (+) LPS||64.9||285.1
| Eric||24 hr (+) LPS||64.9||285.1
|-
|-
| 48 hr (+) LPS||74.7||275.3
| Eric||48 hr (+) LPS||74.7||275.3
|-
|-
| 72 hr (+) LPS||97.9||252.1
| Eric||72 hr (+) LPS||97.9||252.1
|-
|-
| 24 hr (+) IFNg||34.9||315.1
| Eric||24 hr (+) IFNg||34.9||315.1
|-
|-
| 48 hr (+) IFNg||40.3||309.7
| Eric||48 hr (+) IFNg||40.3||309.7
|-
|-
| 72 hr (+) IFNg||350.0||0.0
| Eric||72 hr (+) IFNg||350.0||0.0
|-
|-
| 24 hr (+) LPS (+) IFNg||92.6||257.4
| Sunny||24 hr (+) LPS (+) IFNg||92.6||257.4
|-
|-
| 48 hr (+) LPS (+) IFNg||128.8||221.2
| Sunny||48 hr (+) LPS (+) IFNg||128.8||221.2
|-
|-
| 72 hr (+) LPS (+) IFNg||62.5||287.5
| Sunny||72 hr (+) LPS (+) IFNg||62.5||287.5
|-
|-
| 24 hr control||34.2||315.8
| Sunny||24 hr control||34.2||315.8
|-
|-
| 48 hr control||35.6||314.4
| Sunny||48 hr control||35.6||314.4
|-
|-
| 72 hr control||34.2||315.8
| Sunny||72 hr control||34.2||315.8
|}
|}
* I am student A, Sunny is student B. Therefore, the tubes are divided as follows:


==Part Two: 8% Acrylamide Gel==
==Part Two: 8% Acrylamide Gel==

Revision as of 13:01, 17 March 2009

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Experimental Goals

  1. Perform an Immunoprecipitation of iNOS/NOS2 from LPS +/- IFNg stimulated cell lysates.
  2. Preparation of 8% PAGE running gel.

Part One: IP of iNOS/NOS2

  • Volumes of RIPA buffer required for IP. (See previous entry for calculation of volumes.)
  • I am student A, Sunny is Student B, therefore the samples are divided as follows:
Experimenter Sample Volume of sample required (µL) Volume of RIPA buffer needed (µL)
Eric 24 hr (+) LPS 64.9 285.1
Eric 48 hr (+) LPS 74.7 275.3
Eric 72 hr (+) LPS 97.9 252.1
Eric 24 hr (+) IFNg 34.9 315.1
Eric 48 hr (+) IFNg 40.3 309.7
Eric 72 hr (+) IFNg 350.0 0.0
Sunny 24 hr (+) LPS (+) IFNg 92.6 257.4
Sunny 48 hr (+) LPS (+) IFNg 128.8 221.2
Sunny 72 hr (+) LPS (+) IFNg 62.5 287.5
Sunny 24 hr control 34.2 315.8
Sunny 48 hr control 35.6 314.4
Sunny 72 hr control 34.2 315.8

Part Two: 8% Acrylamide Gel


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