User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/24: Difference between revisions
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====Standard Curve==== | ====Standard Curve==== | ||
[[Image:090324_ELISA_Standard_Curve.png|800px]] | [[Image:090324_ELISA_Standard_Curve.png|800px]] | ||
====Calculated Concentration of TNF-alpha==== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Samples''' | |||
| align="center" style="background:#f0f0f0;"|'''Average Absorbance (A450)''' | |||
| align="center" style="background:#f0f0f0;"|'''100X Average Absorbance (A450)''' | |||
| align="center" style="background:#f0f0f0;"|'''Calculated [TNFa] (pg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Normalized [TNFa] (pg/mL)''' | |||
|- | |||
| 0 hr PBS control||0.08575||8.575||-342.364532||0 | |||
|- | |||
| 30 min +LPS||0.09725||9.725||-285.7142857||0 | |||
|- | |||
| 60 min +LPS||0.102||10.2||-262.3152709||0 | |||
|- | |||
| 90 min +LPS||0.10525||10.525||-246.3054187||0 | |||
|- | |||
| Supplied 6 hr +LPS sample||0.404||40.4||1225.369458||1225.369458 | |||
|- | |||
| Supplied 8 hr +LPS sample||0.13075||13.075||-120.6896552||0 | |||
|- | |||
| 1/5 dilution of Day 1 +LPS stimulation||0.2265||22.65||1754.926108||1754.926108 | |||
|- | |||
| 90 min + IFNg||0.081||8.1||-365.7635468||0 | |||
|- | |||
| Day 1 +PBS control||0.1085||10.85||-230.2955665||0 | |||
|- | |||
| 30 min +LPS +IFNg||0.0855||8.55||-343.5960591||0 | |||
|- | |||
| 60 min +LPS +IFNg||0.09325||9.325||-305.4187192||0 | |||
|- | |||
| 90 min +LPS +IFNg||0.09925||9.925||-275.862069||0 | |||
|- | |||
| Supplied 6 hr +LPS +IFNg||0.20725||20.725||256.1576355||256.1576355 | |||
|- | |||
| Supplied 8 hr +LPS +IFNg||0.17825||17.825||113.3004926||113.3004926 | |||
|- | |||
| 1/5 dilution Day 1 +LPS +IFNg||0.263||26.3||2653.940887||2653.940887 | |||
|- | |||
| Day 1 +IFNg||0.10325||10.325||-256.1576355||0 | |||
|} | |||
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Revision as of 22:37, 31 March 2009
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Goals
Part 1: ELISA Detection of TNFa in LPS +/- IFNg stimulated culture supernatants.96 Well Immunoplate Layout
Notes
Part 2: SDS-PAGEEric's Gel
Sunny's Gel
Notes
Part 3: Western TransferNotes
ResultsELISA Results
Tabluated Average Absorbance Data
Standard CurveCalculated Concentration of TNF-alpha
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