User:Federico Castro M/DSO4: Difference between revisions
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[[Image:Fed6.JPG|right|first devise]] | [[Image:Fed6.JPG|right|first devise]] | ||
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| <!-- Biobrick names --><small>Cl lam</small> || align="center" | <small> luxl</small> || align="center" | <small> luxl</small> ||<font color="white">________</font> <small>luxr</small><font color="white">_________</font><small>luxPL </small>||<font color="white">________</font> <small>tetR</small><font color="white">_________</font><small>tetR </small>||<font color="white">_______</font> <small>Cl lam</small>||<font color="white">_______</font> <small>aiiA</small>|| align="center" | <small>EYFP</small> | |||
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| <!-- Biobrick images --> | [[Image:Prom.JPG]] || [[Image:RCT.JPG]] || [[Image:Prom.JPG]] || [[Image:INV.JPG]]|| [[Image:INV.JPG]] || [[Image:RC_.JPG]] || [[Image:RC_.JPG]] || [[Image:RET.JPG]] | |||
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| <!-- Biobrock numbers -->align="center" bgcolor="lime" | <small><bbpart>R0051</bbpart></small> || align="center" bgcolor="lime" | <small><bbpart>F1610</bbpart></small> ||align="center" bgcolor="lime" | <small><bbpart>J23119</bbpart></small> ||align="center" bgcolor="lime" | <small><bbpart>I1762001</bbpart></small> ||align="center" bgcolor="lime" | <small><bbpart>Q04400</bbpart></small> ||align="center" bgcolor="lime" | <small><bbpart>S0103</bbpart></small> ||align="center" bgcolor="lime" | <small><bbpart>C0260</bbpart></small> ||align="center" bgcolor="lime" | <small><bbpart>E0430</bbpart></small> | |||
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I want LuxR to be present on the cells all the time so I in the construction it's regulated by a constitutive promoter but it seems that C0062 don't have LVA tag... the data at the registry for this biobrick is confusing but if that part actually don't have the tag I would be able to put it under the control of Cl without any problem. | I want LuxR to be present on the cells all the time so I in the construction it's regulated by a constitutive promoter but it seems that C0062 don't have LVA tag... the data at the registry for this biobrick is confusing but if that part actually don't have the tag I would be able to put it under the control of Cl without any problem. | ||
Revision as of 17:42, 10 February 2008
Return to the diffusible signal oscillator
Last updated January/21/2008
Design
This devise is very simple and I bet it will be very robust. This is the oscillator I'm developing now for the iGEM 2008. You might notice that BBa_I1762001 don't exist yet... this is because I intend to assemble it as the first mexican biobrick. The simulation of this will be soon.
| Cl lam | luxl | luxl | ________ luxr_________luxPL | ________ tetR_________tetR | _______ Cl lam | _______ aiiA | EYFP |
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| <bbpart>R0051</bbpart> | <bbpart>F1610</bbpart> | <bbpart>J23119</bbpart> | <bbpart>I1762001</bbpart> | <bbpart>Q04400</bbpart> | <bbpart>S0103</bbpart> | <bbpart>C0260</bbpart> | <bbpart>E0430</bbpart> |
I want LuxR to be present on the cells all the time so I in the construction it's regulated by a constitutive promoter but it seems that C0062 don't have LVA tag... the data at the registry for this biobrick is confusing but if that part actually don't have the tag I would be able to put it under the control of Cl without any problem.
