User:Federico Castro M/DSO4: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 11: | Line 11: | ||
This devise is very simple and I bet it will be very robust. This is the oscillator I'm developing now for the iGEM 2008. You might notice that BBa_I1762001 don't exist yet... this is because I intend to assemble it as the first mexican biobrick. The simulation of this will be soon. | This devise is very simple and I bet it will be very robust. This is the oscillator I'm developing now for the iGEM 2008. You might notice that BBa_I1762001 don't exist yet... this is because I intend to assemble it as the first mexican biobrick. The simulation of this will be soon. | ||
[[Image:Fed6.JPG|right|first devise]] | <!-- [[Image:Fed6.JPG|right|first devise]] --> | ||
{| | {| | ||
| <!-- Biobrick names --><small>Cl lam</small> || align="center" | <small> luxl</small> || align="center" | <small> luxl</small> ||<font color="white">________</font> <small>luxr</small><font color="white">_________</font><small>luxPL </small>||<font color="white">________</font> <small>tetR</small><font color="white">_________</font><small>tetR </small>||<font color="white">_______</font> <small>Cl lam</small>||<font color="white">_______</font> <small>aiiA</small>|| align="center" | <small>EYFP</small> | | <!-- Biobrick names --><small>Cl lam</small> || align="center" | <small> luxl</small> || align="center" | <small> luxl</small> ||<font color="white">________</font> <small>luxr</small><font color="white">_________</font><small>luxPL </small>||<font color="white">________</font> <small>tetR</small><font color="white">_________</font><small>tetR </small>||<font color="white">_______</font> <small>Cl lam</small>||<font color="white">_______</font> <small>aiiA</small>|| align="center" | <small>EYFP</small> | ||
Revision as of 17:43, 10 February 2008
Return to the diffusible signal oscillator
Last updated January/21/2008
Design
This devise is very simple and I bet it will be very robust. This is the oscillator I'm developing now for the iGEM 2008. You might notice that BBa_I1762001 don't exist yet... this is because I intend to assemble it as the first mexican biobrick. The simulation of this will be soon.
| Cl lam | luxl | luxl | ________ luxr_________luxPL | ________ tetR_________tetR | _______ Cl lam | _______ aiiA | EYFP |
 |
 |
|
 |
|
 |
|
|
| <bbpart>R0051</bbpart> | <bbpart>F1610</bbpart> | <bbpart>J23119</bbpart> | <bbpart>I1762001</bbpart> | <bbpart>Q04400</bbpart> | <bbpart>S0103</bbpart> | <bbpart>C0260</bbpart> | <bbpart>E0430</bbpart> |
I want LuxR to be present on the cells all the time so I in the construction it's regulated by a constitutive promoter but it seems that C0062 don't have LVA tag... the data at the registry for this biobrick is confusing but if that part actually don't have the tag I would be able to put it under the control of Cl without any problem.
