User:Fermenter User/Notebook/DOE Workshop 20110405: Difference between revisions

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*[[User:Swati_Yewlakar|Swati Yewlakar]]: Postdoctoral Fellow, Department of Chemical and Biological Engineering
*[[User:Swati_Yewlakar|Swati Yewlakar]]: Postdoctoral Fellow, Department of Chemical and Biological Engineering
*[[User:Tacky_Loonchanta|Tacky Loonchanta]]: PhD Student, Department of Chemistry (Burtnick lab)<-- Decision pending
*[[User:Tacky_Loonchanta|Tacky Loonchanta]]: PhD Student, Department of Chemistry (Burtnick lab)<-- Decision pending
<br>


==Off-line Measurement==
==Off-line Measurement==

Revision as of 10:55, 22 March 2011

WORK B1


1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop

Teaching:


When

April 5th - April 14th 2011

Where

LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332

Attending:

  • Louise Creagh: Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular Biophysics
  • Raymond Pan: PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)
  • Swati Yewlakar: Postdoctoral Fellow, Department of Chemical and Biological Engineering
  • Tacky Loonchanta: PhD Student, Department of Chemistry (Burtnick lab)<-- Decision pending


Off-line Measurement

Day Group Run Temp DO pH Day OD Media Day D-1 D-2 D-3 ' Cell growth Phage Yield of Phage
-1 I 1 28.5 70.0 6.5 0 (OD= ) ( ml) 1 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
-1 I 2 28.5 70.0 10.7 0 (OD= ) ( ml) 1 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
-1 I 3 37.0 100.0 4.0 0 (OD= ) ( ml) 1 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
1 A 4 14.2 70.0 6.5 2 (OD= ) ( ml) 3 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
1 A 5 28.5 70.0 6.5 2 (OD= ) ( ml) 3 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
1 A 6 20.0 100.0 4.0 2 (OD= ) ( ml) 3 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
2 B 7 28.5 120.5 6.5 3 (OD= ) ( ml) 4 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
2 B 8 20.0 100.0 9.0 3 (OD= ) ( ml) 4 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
2 B 9 20.0 40.0 4.0 3 (OD= ) ( ml) 4 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
3 A 10 28.5 70.0 6.5 4 (OD= ) ( ml) 5 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
3 A 11 42.8 70.0 6.5 4 (OD= ) ( ml) 5 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
3 A 12 37.0 100.0 9.0 4 (OD= ) ( ml) 5 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
6 B 13 28.5 70.0 6.5 7 (OD= ) ( ml) 8 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
6 B 14 28.5 19.5 6.5 7 (OD= ) ( ml) 8 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
6 B 15 20.0 40.0 9.0 7 (OD= ) ( ml) 8 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
7 A 16 28.5 70.0 2.3 8 (OD= ) ( ml) 9 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
7 A 17 37.0 40.0 9.0 8 (OD= ) ( ml) 9 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
7 A 18 28.5 70.0 6.5 8 (OD= ) ( ml) 9 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
8 B 19 37.0 40.0 4.0 9 (OD= ) ( ml) 10 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
8 B 20 28.5 70.0 6.5 9 (OD= ) ( ml) 10 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)

Day 2 (April 6th, Wed)

<F1,F2,F3>
9:00 <Ann>       Colony check

<F4,F5,F6>
07:30 <Sung-Hye> Culture high density K19 cells
08:00 <Sung-Hye> Prepare dilution tubes
09:00 <Group A>  Sampling
09:30 <Ann>      Phage dilution
09:50 <Ann>      Measure OD of HD cells
10:00 <Ann>      Dry Plates
10:30 <Ann>      Infect K19 cells
11:10 <Ann>      Spot titering

<F7,F8,F9>
09:30 <Group B>  Assembly bioreactor
09:50 <Group B>  Autoclave
11:10 <Group B>  Take out bioreactors from Autoclave
11:10 <Group B>  Install bioreactor
15:30 <Sung-Hye> Thaw stock
16:30 <Sung-Hye> Pre-culture for HD K19 cells
17:00 <Sung-Hye> Inoculation

10:00 - 11:00 Presentation (Rm 4.303)
              # Fermentation Database and Websites
              # How to Install Bioreactors
              # How to setup bio-controllers

Helful links

  1. http://meta.wikimedia.org/wiki/Table
  2. http://excel2wiki.net/ <--- Use this (Copy and Paste Excel to wiki converter)!





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