User:Floriane Briere/Notebook/CHEM-496/2012/02/21

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Objective

Today's objective is to perform a UV-Vis spectroscopy and a fluorescence spectroscopy on the three solutions we have been preparing last 2 weeks. The main purpose of these measures is to quantify the amount of dye and Gold NPs in each solution. The UV-Vis spectroscopy is going to allow us to quantify Gold NPs in each solution, using the fact that Gold NPs absorb at 550nm. To do so, we are going to make a spectrum of the solution by using a spectrophotometer (UV-1800 Shimadzu) in the wavelength range of 200-800nm. The fluorescence spectroscopy is going to allow us to quantify the amount of tagged dye in each solution. We are going to use the fact that our dye absorbs at 602nm and emits at 672nm.

Protocol

  • Preparation of the BSA control solution (the protocol is the same as on the 2/8/12):
  1. 8mL of water + 1mL of HCl (2.84mM) + 1mL of BSA (17.7µM)
  2. 2 hours in the oven (80°C)
  • Finish the dialysis:
  1. At the end of the dialysis we obtained as solution:
    1. For the control solution, a pink solution.
    2. For the 70 ratio solution, a pink solution and lots of small purple fibers.
    3. For the 166 ratio solution, a pink solution and one big purple agregats.
  2. We centrifuged the 70 and 166 solutions (5 minutes at 13200 tpm).

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

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