User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/29: Difference between revisions

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==Entry title==
==29 May 2013==
* Insert content here...
*Seeded GFP/L4440 plates
      -conc. from 10mL LB to 1.5mL
      -blinded for adam: L4440=squiggles, GFP=wiggles
*Performed restreak of GFP/L4440 plate using left over clone inoculate from plate seeding
*was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
*Made M9 solution:
      -Per 1 L of M9:
 
            KH2PO4  3g
            Na2HPO4  6g
            NaCl      5g
 
      Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
      add 1mL of MgSO4 sterily




Revision as of 15:19, 29 May 2013

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29 May 2013

  • Seeded GFP/L4440 plates
     -conc. from 10mL LB to 1.5mL
     -blinded for adam: L4440=squiggles, GFP=wiggles
  • Performed restreak of GFP/L4440 plate using left over clone inoculate from plate seeding
  • was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
  • Made M9 solution:
     -Per 1 L of M9:

           KH2PO4   3g
           Na2HPO4   6g
           NaCl      5g

     Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
     add 1mL of MgSO4 sterily



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