User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/29: Difference between revisions
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*was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth | *was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth | ||
*Made M9 solution: | *Made M9 solution: | ||
Per 1 L of M9: Adjusted for 700mL | |||
-KH2PO4 3g 2.1g | |||
-Na2HPO4 6g 4.2g | |||
-NaCl 5g 3.5g | |||
-H2O to 1L water to 700mL | |||
-read below 700µL MgSO4 | |||
Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave) | Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave) | ||
add 1mL of MgSO4 sterily | add 1mL of MgSO4 sterily | ||
Revision as of 15:30, 29 May 2013
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29 May 2013
-conc. from 10mL LB to 1.5mL -blinded for adam: L4440=squiggles, GFP=wiggles
Per 1 L of M9: Adjusted for 700mL -KH2PO4 3g 2.1g -Na2HPO4 6g 4.2g -NaCl 5g 3.5g -H2O to 1L water to 700mL -read below 700µL MgSO4 Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave) add 1mL of MgSO4 sterily
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