User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/29

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(29 May 2013)
(29 May 2013)
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*Made M9 solution:
*Made M9 solution:
       Per 1 L of M9:              Adjusted for 700mL
       Per 1 L of M9:              Adjusted for 700mL
-
       -KH2PO4   3g               2.1g
+
       -KH2PO4       3g             2.1g
-
       -Na2HPO4   6g            4.2g
+
       -Na2HPO4       6g            4.2g
-
       -NaCl     5g                 3.5g
+
       -NaCl         5g             3.5g
       -H2O to 1L                  water to 700mL
       -H2O to 1L                  water to 700mL
-
       -read below                 700µL MgSO4
+
       -read below                 700µL MgSO4
       Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
       Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)

Revision as of 17:32, 29 May 2013

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29 May 2013

  • Seeded GFP/L4440 plates
     -conc. from 10mL LB to 1.5mL
     -blinded for adam: L4440=squiggles, GFP=wiggles
  • Performed restreak of GFP/L4440 plate using left over clone inoculate from plate seeding
  • was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
  • Made M9 solution:
     Per 1 L of M9:               Adjusted for 700mL
     -KH2PO4        3g             2.1g
     -Na2HPO4       6g             4.2g
     -NaCl          5g             3.5g
     -H2O to 1L                   water to 700mL
     -read below                  700µL MgSO4
     Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
     add 1mL of MgSO4 sterily 
           



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