User:Helen L. Slucher/Notebook/CHEM-572/2014/02/25: Difference between revisions

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* Make de-gassed buffer
* Make de-gassed buffer


==Procedure==
==Dopamine==
* Make tubes with three dopamine solutions
* Make tubes with three dopamine solutions
# Gray (bad)
# Gray (bad)
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* Add HRP or hemoglobin to each tube
* Add HRP or hemoglobin to each tube


* Make buffer with Tris
==Buffer==
* Make buffer with Tris and NaDDT
# Tris
# Tris and NaDDT


==Notes==
==Notes==

Revision as of 11:13, 25 February 2014

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Objective

  • Make 300mL GNPs
  • Make more dopamine
  • Make de-gassed buffer

Dopamine

  • Make tubes with three dopamine solutions
  1. Gray (bad)
  2. Gray (bad)
  3. Purple (good)
  • Add HRP or hemoglobin to each tube

Buffer

  1. Tris
  2. Tris and NaDDT

Notes

  • HRP ---- acetate buffer
  • Dopamine tubes with proteins will be left to react overnight