User:James C. Schwabacher/Notebook/CHEM-571/2013/08/28: Difference between revisions
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Adapted from [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 | Prof. Hartings]] | Adapted from [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 | Prof. Hartings]] | ||
== | ==Procedure== | ||
'''Part I: Synthesizing BSA-AuNP''' | |||
The procedure followed was taken from this [http://pubs.acs.org/doi/abs/10.1021/jp110296y reference]. '''Note''': Instead of adding distilled water to the 10 mL mark on the volumetric flask, a pipette was used to measure out the exact volume of water required to make the total soultion 10 mL. | |||
The gold stock solution had a concentration of 2.54 mM. Thus, in a 10 mL solution, the concentration of gold particles was calculated to be .254 mM. | |||
A BSA concentration of 90 times less than the concentration of gold particles in the final solution was calculated to be 2.82 <math>mu</math>M. | |||
To achieve a final BSA concentration of 2.82 <math>mu</math>M in 10 mL, a 1.81 mL volume of the 15.6 <math>mu</math>M BSA stock solution was used. | |||
'''Part II: Synthesizing Citrate-AuNP''' | |||
==Data== | ==Data== |
Revision as of 13:29, 28 August 2013
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ObjectiveSynthesize two different sets of gold nanoparticles:
Adapted from Prof. Hartings ProcedurePart I: Synthesizing BSA-AuNP The procedure followed was taken from this reference. Note: Instead of adding distilled water to the 10 mL mark on the volumetric flask, a pipette was used to measure out the exact volume of water required to make the total soultion 10 mL. The gold stock solution had a concentration of 2.54 mM. Thus, in a 10 mL solution, the concentration of gold particles was calculated to be .254 mM. A BSA concentration of 90 times less than the concentration of gold particles in the final solution was calculated to be 2.82 [math]\displaystyle{ mu }[/math]M. To achieve a final BSA concentration of 2.82 [math]\displaystyle{ mu }[/math]M in 10 mL, a 1.81 mL volume of the 15.6 [math]\displaystyle{ mu }[/math]M BSA stock solution was used. Part II: Synthesizing Citrate-AuNP Data
NotesThis area is for any observations or conclusions that you would like to note.
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