User:Jarle Pahr/Library preparation
Notes on preparation of nucleic acid libraries:
Kit | Hands-on time | Total time | Supplier | Platforms | DNA requirement | Link |
---|---|---|---|---|---|---|
TruSeq | ||||||
Nextera | ||||||
Moleculo | ||||||
NEBNext | ? | NEB | ||||
Ion AmpliSeq |
Illumina
Truseq: Illumina TruSeq DNA sample preparation guide: http://nextgen.mgh.harvard.edu/attachments/TruSeq_DNA_SamplePrep_Guide_15005180_A.pdf
Nextera:
Nextera DNA sample preparation kits: http://www.illumina.com/products/nextera_dna_sample_prep_kit.ilmn
Nextera sample preparation guide: http://supportres.illumina.com/documents/myillumina/2a3297c5-8a34-4fc5-a148-3e16666fd65e/nextera_dna_sample_prep_guide_15027987_b.pdf
Nextera DNA sample preparation best practices video: http://support.illumina.com/sequencing/sequencing_kits/nextera_dna_kit/training.ilmn
Nextera DNA sample preparation course video: http://support.illumina.com/sequencing/sequencing_kits/nextera_dna_kit/training.ilmn
Moleculo:
PacBio
PacBio SMRT sample prep: http://www.smrtcommunity.com/SampleNet/Sample-Prep
454
Ion torrent
http://integenx.com/wp-content/uploads/2012/07/PrepX-PGM_flyer-IT.pdf
http://www.invitrogen.com/1/3/library-prep-for-sequencing-ion
Ion AmpliSeq library kit 2.0: https://products.invitrogen.com/ivgn/product/4475345