User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/02/25: Difference between revisions
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== | ==Objective== | ||
* | * Today we will be spinning down samples of 30:1 lysozyme Au-Np with 1M salts added, and then UV-Vis will be run on them. After this, the samples with the salts will be heated overnight at 37°C. | ||
After this, we will image the results of the 30:1, 55:1, and 110:1 lysozyme Au-Nps synthesized with salt added before heating. Then we will run atomic absorption of 30:1, 55:1 and 110:1 lysozyme AuNPs synthesized with salts before heating. | |||
== | ==Procedure== | ||
Atomic Absorance of Lysozyme with KCl, NaCl, | ===Atomic Absorption=== | ||
'''Creating the Gold Stock Solutions''' | |||
# Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. | |||
# Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. | |||
# Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. | |||
# Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. | |||
# Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. | |||
'''Atomic Absorption Samples''' | |||
Solutions with the following Au:lysozyme ratio were run on the AA: | |||
* 30:1 MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl | |||
* 55:1 MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl | |||
* 110:1 MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl | |||
==Images== | |||
===Atomic Absorance of Lysozyme with KCl, NaCl, CaCl<sub>2</sub>, MgCl<sub>2</sub>=== | |||
''Figure 1. Gold:protein Ratio to Concentration of Lysozyme-AuNP Baked with Salts'' | |||
[[Image:Lysozyme Gold-protein to Concentration with Salts, JAC.png|550px|]] | |||
===UV-Vis For Solutions that Contained Salt Before Lysozyme AuNP Synthesis=== | |||
[[Image:Screen_shot_2014-02-25_at_11.48.04_PM.png]] | |||
[[Image:Screen_shot_2014-02-25_at_11.47.52_PM.png]] | |||
[[Image:Screen_shot_2014-02-25_at_11.47.42_PM.png]] | |||
[[Image:Screen_shot_2014-02-25_at_11.47.31_PM.png]] | |||
===UV-Vis For Select Variables at More Refined Concentrations=== | |||
[[Image:Screen_shot_2014-02-25_at_11.47.14_PM.png]] | |||
[[Image:Screen_shot_2014-02-25_at_11.46.46_PM.png]] | |||
[[Image:Screen_shot_2014-02-25_at_11.46.17_PM.png]] | |||
[[Image:Screen_shot_2014-02-25_at_11.45.14_PM.png]] | |||
[[Image:Screen_shot_2014-02-25_at_11.45.00_PM.png]] | |||
[[Image:Screen_shot_2014-02-25_at_11.44.28_PM.png]] | |||
[[Image:Screen_shot_2014-02-25_at_11.44.01_PM.png]] | |||
==Notes== | |||
[[Image:notes.2.25.jac.png|700px|]] | |||
These are the results that sum up the samples from last Wednesday with the addition of the variables to 1mL aliquots of 30:1 ratio with salts added after | |||
[[Image:more.2.25.notes.png|500px|]] | |||
These results detail the reaction of different ratios synthesized with salts before heating | |||
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Revision as of 16:06, 16 March 2014
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Objective
After this, we will image the results of the 30:1, 55:1, and 110:1 lysozyme Au-Nps synthesized with salt added before heating. Then we will run atomic absorption of 30:1, 55:1 and 110:1 lysozyme AuNPs synthesized with salts before heating. ProcedureAtomic AbsorptionCreating the Gold Stock Solutions
Solutions with the following Au:lysozyme ratio were run on the AA:
ImagesAtomic Absorance of Lysozyme with KCl, NaCl, CaCl2, MgCl2Figure 1. Gold:protein Ratio to Concentration of Lysozyme-AuNP Baked with Salts UV-Vis For Solutions that Contained Salt Before Lysozyme AuNP SynthesisUV-Vis For Select Variables at More Refined ConcentrationsNotesThese are the results that sum up the samples from last Wednesday with the addition of the variables to 1mL aliquots of 30:1 ratio with salts added after These results detail the reaction of different ratios synthesized with salts before heating
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