User:Jessica Perez/CloningWorkflow
Planning
Before you start any cloning in the lab you first do the following:
- Decide what parts you need
- Design primers
- Draw up your expected plasmid. This can be done in Word or freely avalible DNA editor software such as <html>
<body> <a href="http://biologylabs.utah.edu/jorgensen/wayned/ape/"> APE</a> </body> </html>.
Source of DNA
{ "class": "go.TreeModel",
"nodeDataArray": [
{"key":"1", "name":"Corrado 'Junior' Soprano", "title":"The Boss"}, {"key":"2", "parent":"1", "name":"Tony Soprano", "title":"Underboss"}, {"key":"3", "parent":"1", "name":"Herman 'Hesh' Rabkin", "title":"Advisor"}, {"key":"4", "parent":"2", "name":"Paulie Walnuts", "title":"Capo"}, {"key":"5", "parent":"2", "name":"Ralph Cifaretto", "title":"Capo MIA"}, {"key":"6", "parent":"2", "name":"Silvio Dante", "title":"Consigliere"}, {"key":"7", "parent":"2", "name":"Bobby Baccilien", "title":"Capo"}, {"key":"8", "parent":"4", "name":"Sal Bonpensiero", "title":"MIA"}, {"key":"9", "parent":"4", "name":"Christopher Moltisanti", "title":"Made Man"}, {"key":"10", "parent":"4", "name":"Furio Giunta", "title":"Muscle"}, {"key":"11", "parent":"4", "name":"Patsy Parisi", "title":"Accountant"}
]
}
