User:Jose Fabricio Lopez/Notebook/GEDF/Protocols: Difference between revisions
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(New page: __TOC__ =Protocols= ==DNA== ===Transformation===) |
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=Protocols= | =Protocols= | ||
==DNA== | ==DNA== | ||
===Transformation=== | ===Transformation for E. Coli by electrophoration=== | ||
'''Reagent and instruments''' | |||
*LB medium Liquid | |||
*LB medium Solid | |||
*DNA of plasmid. (10μL) | |||
*Electrocompetent cells. | |||
*Ice. | |||
*Deionized water. | |||
*Paper for deinization. | |||
*Antibiotic | |||
====''Instrucctions''==== | |||
1.-Introduce cells in DNA solution. | |||
2.-Put cells in ice. | |||
3.-Select Cuvettes for electroporation and put voltage. Wait until sound occurs. | |||
4.-Put 500μL of LB liquid (without antibiotic). | |||
5.-Grow up cells by 1 hour at 37°C mixing. | |||
6.-Petri dishes with antibiotic. | |||
7.-Plate cells. | |||
'''Remember:''' Use controls for medium and cells. | |||
Revision as of 16:51, 9 January 2012
Protocols
DNA
Transformation for E. Coli by electrophoration
Reagent and instruments
- LB medium Liquid
- LB medium Solid
- DNA of plasmid. (10μL)
- Electrocompetent cells.
- Ice.
- Deionized water.
- Paper for deinization.
- Antibiotic
Instrucctions
1.-Introduce cells in DNA solution.
2.-Put cells in ice.
3.-Select Cuvettes for electroporation and put voltage. Wait until sound occurs.
4.-Put 500μL of LB liquid (without antibiotic).
5.-Grow up cells by 1 hour at 37°C mixing.
6.-Petri dishes with antibiotic.
7.-Plate cells.
Remember: Use controls for medium and cells.
