User:Jose Fabricio Lopez/Notebook/GEDF/Protocols
Transformation for E. Coli by electrophoration
Reagent and instruments
- LB medium Liquid
- LB medium Solid
- DNA of plasmid. (10μL)
- Electrocompetent cells.
- Deionized water.
- Paper for deinization.
1.-Introduce cells in DNA solution.
2.-Put cells from REVCO in ice and Cuvettes too.
3.-Select Cuvettes for electroporation and put voltage according with the cuvette. Wait until sound occurs.
4.-Put 500μL of LB liquid (without antibiotic).
5.-Grow up cells by 1 hour at 37°C mixing.
6.-Petri dishes with antibiotic.
Remember: Use controls for medium and cells.