User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/09: Difference between revisions
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==WetLab== | ==WetLab== | ||
===Plasmid extraction pBBRmcS5. From DH5α.=== | |||
** | **First lane control | ||
**Plasmids 7 lanes. | **Plasmids 7 lanes. | ||
[[Image:9Mayo2011.pBBRmcS5.Extracción..JPG]] | [[Image:9Mayo2011.pBBRmcS5.Extracción..JPG]] | ||
===Plasmid digestion=== | |||
*7 digestions with HindIII | |||
**2μL 10X Buffer 2 NE | |||
**8μL H<sub>2</sub>0 | |||
**10μL DNA Plasmid stock | |||
**1μL Hind III. | |||
*Marks, | |||
**1 tube without DNA | |||
**1 tube without HindIII | |||
**7 tubes with each plasmid stock. | |||
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Revision as of 11:45, 1 July 2011
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WetLabPlasmid extraction pBBRmcS5. From DH5α.
Plasmid digestion
|