User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/17: Difference between revisions
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== | ==WetLab== | ||
* | * Extracting DNA from 2010 distribution. | ||
*Plate 1. 7A. Plasmid pSB1T3 with 10μL H<sub>2</sub>O millinQ | |||
***Transform DH5α competent cells. | |||
***1.-Cells defrosted on ice. | |||
***2.-100μL of cells for 3μL of DNA from plate. | |||
***3.-3 tubes were generated and transformed. 1 control with 50μL of cells. | |||
***4.-20 minutes on ice. | |||
***5.-Thermal shock at 42°C, 1 minute. | |||
***6.-5 minutes on ice. | |||
***7.-Add 1 ml of LB at each tube. | |||
***8.-1 hour at 37°C. | |||
***9.-Plate on Tet30-LB medium | |||
***Odd cells stored at 4°C. | |||
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Revision as of 05:11, 18 May 2011
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