User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/26: Difference between revisions
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(Autocreate 2011/05/26 Entry for User:Jose_Fabricio_Lopez/Notebook/Logbook) |
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== | ==General== | ||
* | * Meeting with our principal instructor Miguel Ramírez | ||
==WetLab== | |||
*Plasnud extraction of pBBRmcS5 of DH5α. | |||
**Roche's protocol | |||
NOTES: Wash Buffer I used in protocol even when DH5α has low level of endonuclease activity. This is because we are looking for better ligation whit hopefully [http://openwetware.org/wiki/User:Daniel_Ramirez/Notebook/UNAM_Genomics_Mexico_2011/2011/05/25 PCR product] after digestion with XbaI and HindIII. | |||
*Electrophoresis gel of: | |||
**[http://openwetware.org/wiki/User:Daniel_Ramirez/Notebook/UNAM_Genomics_Mexico_2011/2011/05/25 PCR product] with 80V 90min, 2%. | |||
**Today's plasmid extraction. 80V, 90 min, 1%. | |||
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__NOTOC__ | __NOTOC__ | ||
Revision as of 15:59, 26 May 2011
 iGEM 2011
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General
WetLab
NOTES: Wash Buffer I used in protocol even when DH5α has low level of endonuclease activity. This is because we are looking for better ligation whit hopefully PCR product after digestion with XbaI and HindIII.
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