User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/28: Difference between revisions

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==Entry title==
==WetLab==
* Insert content here...
*Plasmid extraction again! of DH5α, this time trying to get better concentration.
**Roche´s kit. Elution buffer: just 40μL.


*Electrophoresis gel of:
**PCR product with 80V, 90min, 2%.
**Today's plasmid extraction. 80V, 90 min, 1%.
Picture not available.
==Digestion==
*4 double (XbaI & HindIII) digestions were performed, with [[User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/05/27 | Daniel´s extraction]] and mine extracted today.


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{| border="1"
!
!1
!2
!3
!4
!5 Control
|--
|DNA
|5†
|5‡
|10†
|10‡
|0
|--
|Enzyme HindIII (μL)
|2
|2
|2
|2
|2
|--
|Enzyme XbaI (μL)
|2
|2
|2
|2
|2
|--
|Buffer 2 10x (NEB)
|2
|2
|2
|2
|2
|--
|BSA
|.2
|.2
|.2
|.2
|.2
|--
|H<sub>2</sub>O
|8.8
|8.8
|3.8
|3.8
|13.8
|}
|}
†Daniel's extraction
‡Fabricio's extraction


__NOTOC__
__NOTOC__

Revision as of 11:10, 30 May 2011

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WetLab

  • Plasmid extraction again! of DH5α, this time trying to get better concentration.
    • Roche´s kit. Elution buffer: just 40μL.
  • Electrophoresis gel of:
    • PCR product with 80V, 90min, 2%.
    • Today's plasmid extraction. 80V, 90 min, 1%.

Picture not available.

Digestion


1 2 3 4 5 Control
DNA 5† 5‡ 10† 10‡ 0
Enzyme HindIII (μL) 2 2 2 2 2
Enzyme XbaI (μL) 2 2 2 2 2
Buffer 2 10x (NEB) 2 2 2 2 2
BSA .2 .2 .2 .2 .2
H2O 8.8 8.8 3.8 3.8 13.8

†Daniel's extraction ‡Fabricio's extraction


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