User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/02: Difference between revisions

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(Autocreate 2011/08/02 Entry for User:Jose_Fabricio_Lopez/Notebook/Logbook)
 
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==Entry title==
=Objectives=
* Insert content here...
* Ligation of Plasmid pSB1AK3/BBa_B0015 and Prom+Kanamycin.


==Ligation==


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{|border=1
|--
!
! 5:2
! 4:3
! -Vector
! -Insert
|--
| DNA insert
| 5
| 4
| 5
| 0
|--
| DNA vector
| 2
| 3
| 0
| 3
|--
| Buffer T4 DNA Ligase NEB
| 1
| 1
| 1
| 1
|--
| T4 DNA Ligase NEB
| 1
| 1
| 0
| 0
|--
| H<sub>2</sub>O
| 1
| 1
| 4
| 6
|}
|}


__NOTOC__
I left reaction overnight.
 
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Revision as of 20:40, 2 August 2011

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Objectives

  • Ligation of Plasmid pSB1AK3/BBa_B0015 and Prom+Kanamycin.

Ligation

5:2 4:3 -Vector -Insert
DNA insert 5 4 5 0
DNA vector 2 3 0 3
Buffer T4 DNA Ligase NEB 1 1 1 1
T4 DNA Ligase NEB 1 1 0 0
H2O 1 1 4 6

I left reaction overnight.


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