User:Karlena L. Brown/Notebook/PVOH Research/2013/02/13: Difference between revisions
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# Create folder a to save the information under '''MK''' | # Create folder a to save the information under '''MK''' | ||
==Fluorescence Limit of Detection Calculations== | |||
'''Fluorescent Standards For Limit of Detection: 0.25μM, 0.5μM, 1μM, 92μM, & 165μM''' | '''Fluorescent Standards For Limit of Detection: 0.25μM, 0.5μM, 1μM, 92μM, & 165μM''' | ||
Revision as of 20:06, 8 April 2013
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OBJECTIVES
Fluorescence Analysis Instructions
Fluorescence Limit of Detection CalculationsFluorescent Standards For Limit of Detection: 0.25μM, 0.5μM, 1μM, 92μM, & 165μM 0.25μM Rhodamine 6G Dye Concentration M1V1 = M2V2 0.25μM (RG6)x 5mL = (0.5μM)V2 V2 = 2.5mL 0.5μM Rhodamine 6G Dye Concentration M1V1 = M2V2 0.5μM (RG6)x 10mL = (92μM)V2 V2 = 54.34μL 1μM Rhodamine 6G Dye Concentration M1V1 = M2V2 1μM (RG6)x 25mL = (92μM)V2 V2 = 271.74μL Notes
# Excitation: 480nm # Emission Range: 500-650nm # Excitation Slit Width: 10 # Emission Slit Width: 10 # Scan Speed: 1200 |