User:Karlena L. Brown/Notebook/PVOH Research/2013/03/29: Difference between revisions

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==PVOH Film Preparations==
==OBJECTIVES==
'''Prepared PVOH films in water:'''
* Prepare controlled microsphere samples – PVOH 130K and 146K
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
* Finish decanting microsphere samples prepared '''03/22/13'''
'''(Actual Mass = 0.4934g)'''
* Dry microsphere samples rinsed with hexanes '''(03/27/13)''' in preparation for DSC and X-ray
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
* Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
'''(Actual Mass = 0.9965g)'''
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker


'''Standard PVOH Film Protocol:'''
==PVOH 146K Prepared Microsphere Samples & Dye Preparations 4==
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.


'''Notes:'''
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
* Heat was reduced when solutions began to boil too rapidly
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
 
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''PVOH Type'''
| align="center" style="background:#f0f0f0;"|'''PVOH Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Safflower Oil Added (mL)'''
|-
| 130K||0.10400||20||35
|-
| 140K||0.1022||20||35
|}
 
==Notes==
* Samples previously rinsed with hexanes '''03/27/13''' and set aside to air dry in the fume hood were not fully dry. Therefore, these samples were unable to be run for DSC and X-ray diffraction.
* Also these samples were placed in the lypolizer instrument in attempt to thoroughly dry each sample; however, this was a failed attempt.
* The lypolizer caused the microspheres to melt together and form hydrogels instead of small spherical samples.
* For the controlled microsphere samples steadily being prepared, while forming emulsions each sample maintained a very smooth milky cream colored appearance in comparison to the other microsphere samples prepared containing clay additions.




Revision as of 19:06, 8 April 2013

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OBJECTIVES

  • Prepare controlled microsphere samples – PVOH 130K and 146K
  • Finish decanting microsphere samples prepared 03/22/13
  • Dry microsphere samples rinsed with hexanes (03/27/13) in preparation for DSC and X-ray
  • Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing

PVOH 146K Prepared Microsphere Samples & Dye Preparations 4

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH Type PVOH Mass (g) H2O Added (mL) Safflower Oil Added (mL)
130K 0.10400 20 35
140K 0.1022 20 35

Notes

  • Samples previously rinsed with hexanes 03/27/13 and set aside to air dry in the fume hood were not fully dry. Therefore, these samples were unable to be run for DSC and X-ray diffraction.
  • Also these samples were placed in the lypolizer instrument in attempt to thoroughly dry each sample; however, this was a failed attempt.
  • The lypolizer caused the microspheres to melt together and form hydrogels instead of small spherical samples.
  • For the controlled microsphere samples steadily being prepared, while forming emulsions each sample maintained a very smooth milky cream colored appearance in comparison to the other microsphere samples prepared containing clay additions.



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