User:Karlena L. Brown/Notebook/PVOH Research/2013/03/29

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  • Prepare controlled microsphere samples – PVOH 130K and 146K
  • Finish decanting microsphere samples prepared 03/22/13
  • Dry microsphere samples rinsed with hexanes (03/27/13) in preparation for DSC and X-ray
  • Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing

Mixed PVOH Microsphere Samples & Dye Preparations


1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH Type PVOH Mass (g) H2O Added (mL) Safflower Oil Added (mL)


  • Samples previously rinsed with hexanes 03/27/13 and set aside to air dry in the fume hood were not fully dry. Therefore, these samples were unable to be run for DSC and X-ray diffraction.
  • Also these samples were placed in the lypolizer instrument in attempt to thoroughly dry each sample; however, this was a failed attempt.
  • The lypolizer caused the microspheres to melt together and form hydrogels instead of small spherical samples.
  • For the controlled microsphere samples steadily being prepared, while forming emulsions each sample maintained a very smooth milky cream colored appearance in comparison to the other microsphere samples prepared containing clay additions.

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