User:Karlena L. Brown/Notebook/PVOH Research/2013/03/29: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 12: Line 12:
* Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing
* Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing


==PVOH 146K Prepared Microsphere Samples & Dye Preparations 4==
==Mixed PVOH Microsphere Samples & Dye Preparations==
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''


Revision as of 19:07, 8 April 2013

PVOH Research <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

OBJECTIVES

  • Prepare controlled microsphere samples – PVOH 130K and 146K
  • Finish decanting microsphere samples prepared 03/22/13
  • Dry microsphere samples rinsed with hexanes (03/27/13) in preparation for DSC and X-ray
  • Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing

Mixed PVOH Microsphere Samples & Dye Preparations

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH Type PVOH Mass (g) H2O Added (mL) Safflower Oil Added (mL)
130K 0.10400 20 35
140K 0.1022 20 35

Notes

  • Samples previously rinsed with hexanes 03/27/13 and set aside to air dry in the fume hood were not fully dry. Therefore, these samples were unable to be run for DSC and X-ray diffraction.
  • Also these samples were placed in the lypolizer instrument in attempt to thoroughly dry each sample; however, this was a failed attempt.
  • The lypolizer caused the microspheres to melt together and form hydrogels instead of small spherical samples.
  • For the controlled microsphere samples steadily being prepared, while forming emulsions each sample maintained a very smooth milky cream colored appearance in comparison to the other microsphere samples prepared containing clay additions.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Karlena_L._Brown/Notebook/PVOH_Research/2013/03/29&oldid=689855"